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egfp 2  (ATCC)


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    ATCC egfp 2
    Egfp 2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfp 2/product/ATCC
    Average 95 stars, based on 42 article reviews
    egfp 2 - by Bioz Stars, 2026-03
    95/100 stars

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    Image Search Results


    (A) qRT-PCR analysis validation of E3 ligase knockdown efficiency (shPIRH2, shKCTD11, shMDM2, shCHFR, shUHRF1, shTRIM46) in HeLa cells. Data are mean ± SEM; n = 3. *** P < 0.001. (B) Immunoblotting analysis of HDAC1/2 stability in E3 ligase-knockdown HeLa cells infected with HSV-1 (MOI = 1). (C) Co-IP assay of endogenous HDAC1-MDM2 interaction in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (D) Co-IP assay of FLAG-HDAC1 with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (E) Co-IP assay of FLAG-HDAC1 (WT or K74R) with EGFP-MDM2 in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (F) Ubiquitination assays of FLAG-HDAC1 (WT or K74R) co-expressed with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi).

    Journal: bioRxiv

    Article Title: The targeted cytosolic degradation of class I histone deacetylases is essential for efficient alphaherpesvirus replication

    doi: 10.64898/2026.01.02.697337

    Figure Lengend Snippet: (A) qRT-PCR analysis validation of E3 ligase knockdown efficiency (shPIRH2, shKCTD11, shMDM2, shCHFR, shUHRF1, shTRIM46) in HeLa cells. Data are mean ± SEM; n = 3. *** P < 0.001. (B) Immunoblotting analysis of HDAC1/2 stability in E3 ligase-knockdown HeLa cells infected with HSV-1 (MOI = 1). (C) Co-IP assay of endogenous HDAC1-MDM2 interaction in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (D) Co-IP assay of FLAG-HDAC1 with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (E) Co-IP assay of FLAG-HDAC1 (WT or K74R) with EGFP-MDM2 in HSV-1-infected HeLa cells (MOI = 1; 24 hpi). (F) Ubiquitination assays of FLAG-HDAC1 (WT or K74R) co-expressed with EGFP-MDM2 (WT or ΔRING) in HSV-1-infected HeLa cells (MOI = 1; 24 hpi).

    Article Snippet: Antibodies against CHK1 (25887-1-AP), CHK2 (13954-1-AP), RAD51 (14961-1-AP), β-actin (66009-1-lg), P53 (10442-1-AP), HDAC1 (10197-1-AP), HDAC2 (12922-3-AP), HDAC4 (17449-1-AP), HDAC6 (12834-1-AP), HDAC11 (67949-1-Ig), and EGFP (50430-2-AP) were purchased from Proteintech; antibodies against p-P53 (9286), p-ATM (13050), ATM (2873), ATR (13934), p-ATR (2853), p-CHK1 (12302), p-CHK2 (2197), γ-H2AX (80312), H3 (4499), H4K8ac (2594), H4K12ac (13944), H4 (13919), H2AX (7631), H3K9ac (4658), H3K27ac (8173), UB (20326), and MDM2 (86934) were purchased from Cell Signaling Technology; H3K56ac antibody (07-677) was purchased from Millipore; ICP4 antibody (ab6514) was purchased from Abcam; ICP0 antibody (sc-53070) was purchased from Santa Cruz Biotechnology; FLAG antibody (F7425) was purchased from Sigma-Aldrich; HA antibody (A00169) was purchased from GenScript.

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Knockdown, Western Blot, Infection, Co-Immunoprecipitation Assay, Ubiquitin Proteomics